Composite
Part:BBa_K4137008:Design
Designed by: YA-JHIH KO Group: iGEM22_GEMS_Taiwan (2022-08-20)
mleR expressing construct
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 49
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The T7 promoter enables over production of mleR. RBS (AAGGAG) has been shown to be efficient in protein expression MleR can be regulated by malate acid, which is presented around the roots. MleR will be further used as a signal for the production of antitoixen CcdA. The Myc tag binds to mleR and allows the isolation of mleR for identification of individual concentrations
Source
https://www.snapgene.com/resources/plasmid-files/?set=pet_and_duet_vectors_(novagen)&plasmid=pET-28a(%2B) https://www.snapgene.com/resources/plasmid-files/?set=pet_and_duet_vectors_(novagen)&plasmid=pET-28a(%2B) https://journals.asm.org/doi/10.1128/jb.171.6.3108-3114.1989